abstract：:Electron cryo-microscopy (cryo-EM) of purified macromolecular complexes is now providing 3D-structures at near-atomic resolution (Kühlbrandt, 2014). Cryo-EM can tolerate heterogeneous specimens, however, high-resolution efforts demand highly optimized samples. Therefore, significant pre-screening and evaluation is ess...

journal_title：Journal of structural biology

authors： Gewering T,Januliene D,Ries AB,Moeller A

更新日期：2018-09-01 00:00:00

abstract：:HIV-1 Rev mediates the nuclear export of unspliced and partially-spliced viral transcripts for the production of progeny genomes and structural proteins. In this process, four (or more) copies of Rev assemble onto a highly-structured 351-nt region in such viral transcripts, the Rev response element (RRE). How this occ...

journal_title：Journal of structural biology

authors： Watts NR,Eren E,Zhuang X,Wang YX,Steven AC,Wingfield PT

更新日期：2018-08-01 00:00:00

abstract：:Centrioles are microtubule-based structures that play essential roles in cell division and cilia biogenesis. Cep120 is an important protein for correct centriole formation and mutations in the Cep120 gene cause severe human diseases like Joubert syndrome and complex ciliopathies. Here, we show that Cep120 contains thr...

journal_title：Journal of structural biology

authors： Sharma A,Gerard SF,Olieric N,Steinmetz MO

更新日期：2018-07-01 00:00:00

abstract：:Thebaine 6-O-demethylase (T6ODM) from Papaver somniferum (opium poppy), which belongs to the non-heme 2-oxoglutarate/Fe(II)-dependent dioxygenases (ODD) family, is a key enzyme in the morphine biosynthesis pathway. Initially, T6ODM was characterized as an enzyme catalyzing O-demethylation of thebaine to neopinone and ...

journal_title：Journal of structural biology

authors： Kluza A,Niedzialkowska E,Kurpiewska K,Wojdyla Z,Quesne M,Kot E,Porebski PJ,Borowski T

更新日期：2018-06-01 00:00:00

abstract：:Neuraminidase (NA) inhibitors can suppress NA activity to block the release of progeny virions and are effective against influenza viruses. As potential anti-flu drugs with unique functions, NA inhibitors are greatly concerned by the worldwide scientists. It has been reported recently that one of the novel quindoline ...

journal_title：Journal of structural biology

authors： Guan S,Xu Y,Qiao Y,Kuai Z,Qian M,Jiang X,Wang S,Zhang H,Kong W,Shan Y

更新日期：2018-05-01 00:00:00

abstract：:The protist Trypanosoma brucei is an emerging model for the study of cilia and flagella. Here, we used scanning transmission electron microscopy (STEM) tomography to describe the structure of the trypanosome transition zone (TZ). At the base of the TZ, nine transition fibres irradiate from the B microtubule of each do...

journal_title：Journal of structural biology

authors： Trépout S,Tassin AM,Marco S,Bastin P

更新日期：2018-04-01 00:00:00

abstract：:Polynoid worm elytra emit light when mechanically or electrically stimulated. Specialized cells, the photocytes, contain light emitting machineries, the photosomes. Successive stimulations induce light intensity variations and show a coupling within and between photosomes. Here, we describe, using electron tomography ...

journal_title：Journal of structural biology

authors： Ouldali M,Maury V,Nicolas G,Lepault J

更新日期：2018-04-01 00:00:00

abstract：:Computational protein design methods have enabled the design of novel protein structures, but they are often still limited to small proteins and symmetric systems. To expand the size of designable proteins while controlling the overall structure, we developed Elfin, a genetic algorithm for the design of novel proteins...

journal_title：Journal of structural biology

authors： Yeh CT,Brunette TJ,Baker D,McIntosh-Smith S,Parmeggiani F

更新日期：2018-02-01 00:00:00

abstract：:The structures of the compact and swollen southern bean mosaic virus (SBMV) particles have been compared by X-ray diffraction and proton magnetic resonance (PMR). Small-angle X-ray scattering showed that removal of divalent cations at alkaline pH causes the particle diameter to increase from 289Å in the native SBMV by...

journal_title：Journal of structural biology

authors： Li J,Fricks C,Rayment I,Caspar DLD

更新日期：2017-12-01 00:00:00

abstract：:We consider the problem of estimating an unbiased and reference-free ab initio model for non-symmetric molecules from images generated by single-particle cryo-electron microscopy. The proposed algorithm finds the globally optimal assignment of orientations that simultaneously respects all common lines between all imag...

journal_title：Journal of structural biology

authors： Greenberg I,Shkolnisky Y

更新日期：2017-11-01 00:00:00

abstract：:The fungal pathogen Cryptococcus neoformans is a causative agent of meningoencephalitis in humans. For its pathogenicity, the inositol polyphosphate biosynthetic pathway plays critical roles. Recently, Ipk1 from C. neoformans (CnIpk1) was identified as an inositol 1,3,4,5,6-pentakisphosphate 2-kinase that catalyzes th...

journal_title：Journal of structural biology

authors： Oh J,Lee DG,Bahn YS,Rhee S

更新日期：2017-11-01 00:00:00

abstract：:Sickle hemoglobin (HbS) polymerization initiates in the deoxy state with the binding of hydrophobic patch formed by the isopropyl group of βVal6 residue of a hemoglobin tetramer with the hydrophobic pocket of another tetramer, whose hydrophobic patch binds to the hydrophobic groove of a third molecule. Subsequent elon...

journal_title：Journal of structural biology

authors： Das R,Mitra A,Bhat V,Mandal AK

更新日期：2017-07-01 00:00:00

abstract：:We describe a new implementation for the reconstruction of helical assemblies in the empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for the 3D reconstruction by embedding helical symmetry operators in Fourier-space, and deals with deviations from perfect helical symmetry through...

journal_title：Journal of structural biology

authors： He S,Scheres SHW

更新日期：2017-06-01 00:00:00

abstract：:The skeletal system of Demospongiae consists of siliceous spicules, which are composed of an axial channel containing an organic axial filament (AF) surrounded by a compact layer of hydrated amorphous silica. Here we report the ultrastructural investigations of the AF of siliceous spicules from two Demospongiae: Suber...

journal_title：Journal of structural biology

authors： Werner P,Blumtritt H,Natalio F

更新日期：2017-06-01 00:00:00

abstract：:In Streptomycetes, tetracycline repressor family of transcription regulators (TetR-FTRs) controls various biological processes including antibiotic biosynthesis, cellular morphology and innate resistance. Here, we focus on understanding the structural basis of transcription regulation by CprB, a member of TetR-FTRs fr...

journal_title：Journal of structural biology

authors： Bhukya H,Jana AK,Sengupta N,Anand R

更新日期：2017-05-01 00:00:00

abstract：:We present a sample preparation method for cryo-electron microscopy (cryo-EM) that requires only 3-20nL of sample to prepare a cryo-EM grid, depending on the protocol used. The sample is applied and spread on the grid by a microcapillary. The procedure does not involve any blotting steps, and real-time monitoring allo...

journal_title：Journal of structural biology

authors： Arnold SA,Albiez S,Bieri A,Syntychaki A,Adaixo R,McLeod RA,Goldie KN,Stahlberg H,Braun T

更新日期：2017-03-01 00:00:00

abstract：:Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content an...

journal_title：Journal of structural biology

authors： Grange M,Vasishtan D,Grünewald K

更新日期：2017-02-01 00:00:00

abstract：:While cryo-electron tomography (cryo-ET) can reveal biological structures in their native state within the cellular environment, it requires the production of high-quality frozen-hydrated sections that are thinner than 300nm. Sample requirements are even more stringent for the visualization of membrane-bound protein c...

journal_title：Journal of structural biology

authors： Schaffer M,Mahamid J,Engel BD,Laugks T,Baumeister W,Plitzko JM

更新日期：2017-02-01 00:00:00

abstract：:Enveloped viruses enter their host cells by membrane fusion. The process of attachment and fusion in retroviruses is mediated by a single viral envelope glycoprotein (Env). Conformational changes of Env in the course of fusion are a focus of intense studies. Here we provide further insight into the changes occurring i...

journal_title：Journal of structural biology

authors： Riedel C,Vasishtan D,Siebert CA,Whittle C,Lehmann MJ,Mothes W,Grünewald K

更新日期：2017-02-01 00:00:00

abstract：:Recent advances in cryo-electron tomography (cryo-ET) have allowed direct visualization of the initial interactions between bacteriophages and their hosts. Previous studies focused on phage infection in Gram-negative bacteria but it is of particular interest how phages penetrate the thick, highly cross-linked Gram-pos...

journal_title：Journal of structural biology

authors： Farley MM,Tu J,Kearns DB,Molineux IJ,Liu J

更新日期：2017-02-01 00:00:00

abstract：:Cryo-electron tomography (cryoET) allows 3D structural information to be obtained from cells and other biological samples in their close-to-native state. In combination with subtomogram averaging, detailed structures of repeating features can be resolved. CryoET data is collected as a series of images of the sample fr...

journal_title：Journal of structural biology

authors： Hagen WJH,Wan W,Briggs JAG

更新日期：2017-02-01 00:00:00

abstract：:Lifetimes of bound states of protein complexes or biomolecule folded states typically decrease when subject to mechanical force. However, a plethora of biological systems exhibit the counter-intuitive phenomenon of catch bonding, where non-covalent bonds become stronger under externally applied forces. The quest to un...

journal_title：Journal of structural biology

authors： Chakrabarti S,Hinczewski M,Thirumalai D

更新日期：2017-01-01 00:00:00

abstract：:Chemotactic methyltransferase, CheR catalyse methylation of specific glutamate residues in the cytoplasmic domain of methyl-accepting chemotactic protein receptors (MCPRs). The methylation of MCPRs is essential for the chemical sensing and chemotactic bacterial mobility towards favorable chemicals or away from unfavor...

journal_title：Journal of structural biology

authors： Batra M,Sharma R,Malik A,Dhindwal S,Kumar P,Tomar S

更新日期：2016-12-01 00:00:00

abstract：:Here we report the first crystal structure of a secretory α-glucoside hydrolase isolated from Pseudoalteromonas sp. K8, PspAG97A, which belongs to glycoside hydrolase family 97 and exhibits halophilic property. PspAG97A lacks an acidic surface, that is considered essential for protein stability at high salinity. Inter...

journal_title：Journal of structural biology

authors： He C,Li J,Li W,Xue Y,Fang Z,Fang W,Zhang X,Wang X,Xiao Y

更新日期：2016-12-01 00:00:00

abstract：:As a major player of the innate immune system, surfactant protein D (SP-D) recognizes and promotes elimination of various pathogens such as Gram-negative bacteria. SP-D binds to l-glycero-d-manno-heptose (Hep), a constituent of the partially conserved lipopolysaccharide (LPS) inner core of many Gram-negative bacteria....

journal_title：Journal of structural biology

authors： Reinhardt A,Wehle M,Geissner A,Crouch EC,Kang Y,Yang Y,Anish C,Santer M,Seeberger PH

更新日期：2016-09-01 00:00:00

abstract：:Skeletal elements that are exposed to heavy mechanical loads may provide important insights into the evolutionary solutions to mechanical challenges. We analyzed the microscopic architecture of dactylus claws in the woodlice Porcellio scaber and correlated these observations with analyses of the claws' mineral composi...

journal_title：Journal of structural biology

authors： Vittori M,Srot V,Žagar K,Bussmann B,van Aken PA,Čeh M,Štrus J

更新日期：2016-08-01 00:00:00

abstract：:Macromolecular complexes perform their physiological functions by local rearrangements of their constituents and biochemically interacting with their reaction partners. These rearrangements may involve local rotations and the induction of local strains causing different mechanical efforts and stretches at the differen...

journal_title：Journal of structural biology

authors： Sorzano CO,Martín-Ramos A,Prieto F,Melero R,Martín-Benito J,Jonic S,Navas-Calvente J,Vargas J,Otón J,Abrishami V,de la Rosa-Trevín JM,Gómez-Blanco J,Vilas JL,Marabini R,Carazo JM

更新日期：2016-07-01 00:00:00

abstract：:The optimal balance of cellular nucleotides and the efficient elimination of non-canonical nucleotides are critical to avoiding erroneous mutation during DNA replication. One such mechanism involves the degradation of excessive or abnormal nucleotides by nucleotide-hydrolyzing enzymes. YpgQ contains the histidine-aspa...

journal_title：Journal of structural biology

authors： Jeon YJ,Park SC,Song WS,Kim OH,Oh BC,Yoon SI

更新日期：2016-07-01 00:00:00

abstract：:Magnetotactic bacteria are Gram-negative bacteria that navigate along geomagnetic fields using the magnetosome, an organelle that consists of a membrane-enveloped magnetic nanoparticle. Magnetite formation and its properties are controlled by a specific set of proteins. MamC is a small magnetosome-membrane protein tha...

journal_title：Journal of structural biology

authors： Nudelman H,Valverde-Tercedor C,Kolusheva S,Perez Gonzalez T,Widdrat M,Grimberg N,Levi H,Nelkenbaum O,Davidov G,Faivre D,Jimenez-Lopez C,Zarivach R

更新日期：2016-06-01 00:00:00

abstract：:Cryo-electron microscopy (cryo-EM) of frozen-hydrated preparations of isolated macromolecular complexes is the method of choice to obtain the structure of complexes that cannot be easily studied by other experimental methods due to their flexibility or large size. An increasing number of macromolecular structures are ...

journal_title：Journal of structural biology

authors： Jonić S,Vargas J,Melero R,Gómez-Blanco J,Carazo JM,Sorzano CO

更新日期：2016-06-01 00:00:00

abstract：:Recent studies reveal that mitochondria take substantial responsibility in cellular functions that are closely related to aging diseases caused by degeneration of neurons. These studies emphasize that the membrane and crista morphology of a mitochondrion should receive attention in order to investigate the link betwee...

journal_title：Journal of structural biology

authors： Tasel SF,Mumcuoglu EU,Hassanpour RZ,Perkins G

更新日期：2016-06-01 00:00:00

abstract：:We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations that enhance MBP solu...

journal_title：Journal of structural biology

authors： Bokhove M,Sadat Al Hosseini H,Saito T,Dioguardi E,Gegenschatz-Schmid K,Nishimura K,Raj I,de Sanctis D,Han L,Jovine L

更新日期：2016-04-01 00:00:00

abstract：:This paper describes steps in the single-particle cryo-EM 3D structure determination of membrane proteins in their membrane environment. Using images of the Kv1.2 potassium-channel complex reconstituted into lipid vesicles, we describe procedures for the merging of focal-pairs of exposures and the removal of the vesic...

journal_title：Journal of structural biology

authors： Jensen KH,Brandt SS,Shigematsu H,Sigworth FJ

更新日期：2016-04-01 00:00:00

abstract：:Brain deposition of Aβ in the form of amyloid plaques is a pathological hallmark of Alzheimer's disease. There are two major species of Aβ in the brain: Aβ42 and Aβ40. Although Aβ40 is several-fold more abundant than Aβ42 in soluble form, Aβ42 is the major component of amyloid plaques. Structural knowledge of Aβ42 fib...

journal_title：Journal of structural biology

authors： Gu L,Tran J,Jiang L,Guo Z

更新日期：2016-04-01 00:00:00

abstract：:Polyketides such as the clinically-valuable antibacterial agent mupirocin are constructed by architecturally-sophisticated assembly lines known as trans-acyltransferase polyketide synthases. Organelle-sized megacomplexes composed of several copies of trans-acyltransferase polyketide synthase assembly lines have been o...

journal_title：Journal of structural biology

authors： Gay DC,Wagner DT,Meinke JL,Zogzas CE,Gay GR,Keatinge-Clay AT

更新日期：2016-03-01 00:00:00

abstract：:Tobacco streak virus (TSV), the type member of Ilarvirus genus, is a major plant pathogen. TSV purified from infected plants consists of a ss-RNA genome encapsidated in spheroidal particles with diameters of 27, 30 and 33nm constructed from multiple copies of a single species of coat protein (CP) subunits. Apart from ...

journal_title：Journal of structural biology

authors： Gulati A,Alapati K,Murthy A,Savithri HS,Murthy MR

更新日期：2016-02-01 00:00:00

abstract：:The ability of bacteria to combat oxidative stress is imperative for their survival. The Alkyl hydroperoxide Reductase (AhpR) system, composed of the AhpC and AhpF proteins, is one of the dominant antioxidant defense systems required for scavenging hydrogen peroxide and organic peroxide. Therefore, it is necessary to ...

journal_title：Journal of structural biology

authors： Kamariah N,Nartey W,Eisenhaber B,Eisenhaber F,Grüber G

更新日期：2016-01-01 00:00:00

abstract：:We report a detailed work of composition and location of naturally formed iron biominerals in plant cells tissues grown in iron rich environments as Imperata cylindrica. This perennial grass grows on the Tinto River banks (Iberian Pyritic Belt) in an extreme acidic ecosystem (pH∼2.3) with high concentration of dissolv...

journal_title：Journal of structural biology

authors： Fuente V,Rufo L,Juárez BH,Menéndez N,García-Hernández M,Salas-Colera E,Espinosa A

更新日期：2016-01-01 00:00:00

abstract：:This study probes the early events during lag phase of aggregation of GNNQQNY using all atom MD simulations in explicit solvent. Simulations were performed by varying system size, temperature and starting configuration. Peptides dispersed randomly in the simulation box come together early on in the simulation and form...

journal_title：Journal of structural biology

authors： Srivastava A,Balaji PV

更新日期：2015-12-01 00:00:00

abstract：:Single particle electron cryomicroscopy (cryo-EM) allows for structures of proteins and protein complexes to be determined from images of non-crystalline specimens. Cryo-EM data analysis requires electron microscope images of randomly oriented ice-embedded protein particles to be rotated and translated to allow for co...

journal_title：Journal of structural biology

authors： Zhao J,Brubaker MA,Benlekbir S,Rubinstein JL

更新日期：2015-11-01 00:00:00